生物
感觉神经元
伤害感受器
背根神经节
神经元
神经科学
体感系统
转录组
电池类型
感觉系统
机械感受器
细胞
基因
遗传学
基因表达
伤害
受体
作者
Changlin Li,Kaicheng Li,Dan Wu,Yan Chen,Hao Luo,Jingrong Zhao,Sa-Shuang Wang,Mingming Sun,Yao Lu,Yan-Qing Zhong,Xuye Hu,Rui Hou,Beibei Zhou,Bao Liu,Huasheng Xiao,Xu Zhang
出处
期刊:Cell Research
[Springer Nature]
日期:2015-12-22
卷期号:26 (1): 83-102
被引量:340
摘要
Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion (DRG) by high-coverage single-cell RNA-sequencing (10 950 ± 1 218 genes per neuron) and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors (MHNs). Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors (MNs) expressing BAI1-associated protein 2-like 1 (Baiap2l1). Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap2l1-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.
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