Effects of Wogonin on Proliferation and Cell Cycle of SPC-A-1 Cells

Objective:To investigate the effect of wogonin on the proliferation and cell cycle of the SPC-A-1 cell line.Method: The cultured SPC-A-1 cells were treated with different concentrations of wogonin.The proliferation of SPC-A-1 cells was detected by MTT method.Cell apoptosis and cell cycle were detected by AO + EB straining and flow cytometry.Result:After wogonin administration,SPC-A-1 cells proliferation inhibition ratio and apoptosis ratio increased significantly in a dose-dependent manner.AO + EB straining showed partial cells presented characteristic morphological changes of apoptosis.SPC-A-1 cells showed G0 /G1 phase blockage when the concentration of wogonin was 10,20 mg.L-1,and S phase blockage appeared when the concentration of wogonin was 30,40 mg.L-1.Conclusion:Wogonin can inhibit the proliferation of SPC-A-1 cells and induce apoptosis,also possess the effect of G0 /G1 and S phase blockage and potential antitumor effect.