Development of Loop-mediated isothermal amplification method for detection of Vibrio parahaemolyticus

Objective To develop an assay of loop-mediated isothermal amplification(LAMP) for detection and identification of Vibrio parahaemolyticus in basic laboratory.Methods The target of Vibrio parahaemolyticus was chosen from gyrB gene,four LAMP primers were designed and reaction conditions were optimized.Results: LAMP method was of high specificity for Vibrio parahaemolyticus.Through the amplification of 14 strains,Vibrio vulnificus was positive,and the other strains were negative.And the detection of LAMP method was finished within 60 minutes,and the detection limit was 25 cfu/ml.Totally 120 seafood samples were detected and positive rate was 62.5%.LAMP method was accord with traditional methods.Conclusion Comparing to traditional method,LAMP method is a good one:saving time,lower requirement of laboratory instruments and of good practicability for Vibrio parahaemolyticus detection.