Application and prospect of nucleic acids detection for Ebola virus

Ebola virus(EBOV)causes severe hemorrhagic fever in humans and nonhuman primates with high mortality rates.Zaire ebola virus is the cause of the 2013-2014 Ebola virus epidemic in West Africa. Rapid identification of the virus is urgently required to prevent the spread of the infection. The real-time qutification PCR assay and the loop-mediated isothermal amplification assay(LAMP) have been developed for EBOV detection; the nucleic acid sequence-based amplification assay(NASBA) and the recombinase polymerase amplification assay(RPA) have great potential for diagnosis of EBOV in the field. This review highlights various methods that can be used for a sensitive detection of EBOV nucleic acids.