Preparation PRV,PPV and JEV detection microarray

PRV,PPV and JEV detection microarray have been prepared in this test.The microarray has been fabricated by spotting target genes on animated slide with the best concentration 200 mg/L of each target gene firstly,then the slide dried,hydrated,UV cross-linked and washed.Probes were labelled by PCR with CY3-dCTP to evaluate the qualification of the microarray.The best concentration of the probe was 3 000 μg/L,with the sensitivity of the microarray detection system of 3 μg/L.The results showed that PRV,PPV and JEV can be detected simultaneously by the microarray with high sensitivity and specificity.The microarray can be reutilized for at least 10 times and conserved for at least 4 months.