The expression of Caspase-3 in liver cell apoptosis caused by endotoxin and the antagonized by Cation A in rats

To explore the relationship between apoptosis of liver cells and Caspase-3 in endotoxemia(ET) and antagonistic effect of Cation A(CA) in rats.Seventy-two adult healthy SD rats were randomly divided into 3 groups,including control group,ET group and CA group.Hepatic tissues in three groups were collected for tests at 3rd,4th,8th and 12th hour after treatment.Apoptosis of liver cells were detected by flow cytometry,and Caspase-3 expression was detected by immunohistochemical staining.The results showed that the percentage of apoptosis of liver cells in ET group were significantly higher than that of control and CA group.The percentage of hepatocyte apoptosis of ET group was in an upward trend,however,which was upward trend in the CA group at the 3rd,4th,8th hours time points,and went downward trend at the 12th hour.Caspase-3 expression in ET group was significantly higher than that of CA group.These results suggested that apoptosis and Caspase-3 protein expression in liver cell was positive correlation in endotoxemia,and CA have a significantly antagonistic effect on inflammatory injure through down-regulation of the expression of Caspase-3 pathway in liver,inhibiting liver cell apoptosis