Epimedium is widely distributed in Guizhou,where there are 17 species and 2 variants of medicinal plants of Epimedium. Experiments were conducted for introduction and cultivation of 12 different habitats of medicinal plants of Epimedium in Guizhou. The results showed that after transplantation 12 Epimedium changed mainly in the following aspects: plant height,flowering and fruiting periods,plush,sepals,petals,and anther color. The study provided a further analysis of environmental factors on influences over Epimedium plant traits of change.