Comparative study on extraction of genomic DNA from different parts of Helianthus tuberosus L.

With the stems,young leaves and tubers of Helianthus tuberosus L.as experimental materials,the genome DNA were extracted with modified CTAB method and examined with 0.8% agarose gel electrophoresis.The absorbance value of DNA from these parts was measured by ultraviolet spectrophotometer at A 260 and A 280 and the ratio of A 260 / A 280 was used to determine the DNA purity and concentration.DNA extracted from these parts of Helianthus tuberosus L.was used as the template for ISSR amplification.The results showed the yield of DNA extracted from Helianthus tuberosus L.leaves was the highest,that from the stems was the second,and that from the tubers was the lowest.The purity of DNA extracted from these parts of Helianthus tuberosus L.was similar.The concentration of DNA extracted from Helianthus tuberosus L.leaves was the the largest(163.2 ng·μL-1) followed by that from stems(137.4 ng·μL-1),and that from the tubers was the lowest(95.5 ng·μL-1).The DNA template extracted from different parts of Helianthus tuberosus L.had no significant effect on ISSR amplification,and DNA concentration could achieve the amplification requirements.The DNA quality extracted from above three parts of Helianthus tuberosus L.was good and could be used in subsequent expermients such as digestion and PCR amplification.