Effect of salidroside on the proliferation and [Ca~(2+)]i of rat pulmonary arterial smooth musicle cells under hypoxia

Objective To study the effect of Salidroside on the proliferation, DNA synthesis and intracellular calcium ion concentration(〔Ca 2+〕i) of pulmonary arterial smooth muscle cells (PASMCs) under hypoxia and to explore its possible mechanism in protection against hypoxia. Methods Rat PASMCs were isolated and cultured. MTT to measure the number of cells grown, 3H-TdR incorporation to test the synthesis of DNA, and fura - 3/AM to assay〔Ca 2+〕i were used in the study. Hypoxic culture of PASMCs was established by self-made hypoxic equipment placed in 37 ℃ incubator.Results Salidroside could inhibit the proliferation and DNA synthesis of PASMCs under hypoxia. The fluorescence intensity of PASMCs was increased under hypoxia, while it was decreased after the addition of Salidroside. The absorption value and 3 H-TdR incorporation of PASMCs decreased 28% and 45% more respectively in hypoxia + Salidroside (32×10 -5 mol·L -1) group than in single hypoxia group.The 〔Ca 2+〕i of PASMCs was (148±14) nmol·L -1 in normoxia group, ( 422±24 ) nmol·L -1 in hypoxia group, and ( 298±18 ) nmol·L -1 in hypoxia + Salidroside (64×10 -5 mol·L -1) group.Conclusion Salidroside may inhibit the proliferation and DNA synthesis of pulmonary arterial smooth muscle cells. Its mechanism may be related to the inhibition on 〔Ca 2+〕i of PASMCs