A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice.

IgG-rheumatoid factor (RF), IgM-RF, antidsDNA, anti-ssDNA, and anti-Sm antibody have been detected and have been thought to play an important role on pathogenesis of systemic lupus erythematosus (SLE) . MRL/MP-lpr/lpr (MRL/lpr) mice have been studied widely as experimental animal model for autoimmune disease. IgG-RF, IgM-RF and anti-ds DNA antibody, anti-ss DNA antibody and anti-Sm antibody were titrated by a one-step sandwich enzyme-linked immuno-sorbent assay (ELISA) system in the serum of MRL/ lpr mice. Titer of the autoantibodies was ranged from 4 to 1000 mU.These results disclosed evidences that the new ELISA kits were useful to titrate IgG-RF, IgM-RF and anti-ds DNA antibody, anti-ss DNA antibody and anti-Sm antibody. The assay system could be employed to study on efficacy of therapeutic agents and to analyse pathoetiology of the autoimmune disease in mice.