Study of Extraction of Indirubin from Indigo Naturalis

Objective: To study opticual extraction process of Indirubin from Indigo. Method: Indigo is first treated with 18% chlorhydric acid, second with ethyl acetate, soluted with 95% ethanol, and finally precipitated with 0.1% NaOH. Result: Extraction rate of Indirubin from Indigo naturalis increases by 240 times. Conclusion: The method can be used for production of drops containing Indirubin.