亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Distribution of constitutive (HO-2) and heat-inducible (HO-1) heme oxygenase isozymes in rat testes: HO-2 displays stage-specific expression in germ cells

生物 血红素加氧酶 支持细胞 原位杂交 同工酶 生精小管 精子发生 内科学 内分泌学 基因表达 睾丸 一氧化氮合酶 分子生物学 血红素 生物化学 一氧化氮 基因 医学
作者
James F. Ewing
出处
期刊:Endocrinology [The Endocrine Society]
卷期号:136 (5): 2294-2302 被引量:22
标识
DOI:10.1210/en.136.5.2294
摘要

The heme oxygenase isozymes, HO-1 and HO-2, oxidatively cleave the heme molecule to produce antioxidants, the bile pigments, the gaseous cellular messenger, CO, and iron, a regulator of transferrin, ferritin, and nitric oxide synthase gene expression. HO-1 (hsp32) is a stress-inducible enzyme, whereas HO-2 is constitutively expressed at high levels in the testes and brain. In the present study, using immunohistochemical and in situ hybridization techniques, we report for the first time the cellular distribution of HO-1 and HO-2 in the testes of normal and heat-shocked rats and define a cell-specific expression of the isozymes and a stage-specific expression of HO-2 in the organ. In normal tissue, HO-1 was present at low levels in the Sertoli cells and could not be detected in germ or Leydig cells. HO-2, on the other hand, was most prominently expressed in residual bodies and was not detected in spermatogonia. Modest levels of HO-2 were observed in spermatocytes, spermatids, and select Leydig cells. In contrast, prominent expression of HO-2 messenger RNAs (mRNAs) was detected by in situ hybridization in spermatogonia, as well as spermatocytes, spermatids, and residual bodies of the seminiferous epithelium. The expression pattern of HO-2 protein and transcript in testes of heat-stressed (42 C; 20 min) rats did not differ from that in the control animals, whereas the expression pattern of HO-1 differed from that in the controls, in which distinct populations of Leydig and Sertoli cells displayed intense immunoreactivity. Thermal stress also resulted in an increase (2.8-fold) in the testicular HO-1 mRNA level within 1 h after treatment, followed by a significant increase (32%) in total microsomal heme oxygenase activity 6 h after treatment. Notably, this increase followed a significant depression (36%) in enzyme activity, which was detected 1 h after hyperthermia. The disparity between HO-2 mRNA and protein distribution clearly indicates cell-specific differences in the translational efficiency of HO-2 transcripts. It appears that HO-2 mRNA translation is linked to the maturation and expression of a factor(s) that regulates this process. This, in turn, appears to coincide with sperm development. HO-1 activity, on the other hand, which has a transcriptional component to its regulation, may have a role in maintenance of the conditions required for spermatogenesis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
40秒前
清爽的绫发布了新的文献求助10
44秒前
彭于晏应助希夷采纳,获得10
56秒前
云飞扬完成签到 ,获得积分10
59秒前
rocky15给快来帮帮我的求助进行了留言
59秒前
Huang发布了新的文献求助10
1分钟前
1分钟前
冷静硬币发布了新的文献求助10
1分钟前
rocky15应助快来帮帮我采纳,获得10
1分钟前
连安阳完成签到,获得积分10
2分钟前
2分钟前
rocky15应助messi采纳,获得10
2分钟前
ding应助messi采纳,获得80
2分钟前
PQ完成签到,获得积分10
2分钟前
2分钟前
研友_VZG7GZ应助冷静硬币采纳,获得10
2分钟前
斯文败类应助聪明的老鼠采纳,获得20
2分钟前
messi发布了新的文献求助10
2分钟前
魔山西红柿完成签到 ,获得积分10
2分钟前
华仔应助科研通管家采纳,获得10
3分钟前
3分钟前
3分钟前
快来帮帮我给快来帮帮我的求助进行了留言
3分钟前
3分钟前
冷静硬币完成签到,获得积分10
3分钟前
冷静硬币发布了新的文献求助10
3分钟前
jason完成签到 ,获得积分10
4分钟前
4分钟前
4分钟前
风行发布了新的文献求助10
4分钟前
缪静柏发布了新的文献求助10
4分钟前
秋雪瑶应助phentjn采纳,获得10
5分钟前
热爱科研的人完成签到 ,获得积分10
5分钟前
py999完成签到,获得积分10
5分钟前
李爱国应助suzy-123采纳,获得10
5分钟前
5分钟前
py999发布了新的文献求助30
6分钟前
6分钟前
Wilson完成签到 ,获得积分10
6分钟前
希夷发布了新的文献求助10
6分钟前
高分求助中
Un calendrier babylonien des travaux, des signes et des mois: Séries iqqur îpuš 1036
Sustainable Land Management: Strategies to Cope with the Marginalisation of Agriculture 1000
Corrosion and Oxygen Control 600
Heterocyclic Stilbene and Bibenzyl Derivatives in Liverworts: Distribution, Structures, Total Synthesis and Biological Activity 500
重庆市新能源汽车产业大数据招商指南(两链两图两池两库两平台两清单两报告) 400
Division and square root. Digit-recurrence algorithms and implementations 400
行動データの計算論モデリング 強化学習モデルを例として 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2545500
求助须知:如何正确求助?哪些是违规求助? 2175642
关于积分的说明 5600159
捐赠科研通 1896337
什么是DOI,文献DOI怎么找? 946234
版权声明 565355
科研通“疑难数据库(出版商)”最低求助积分说明 503557