氟虫腈
背主动脉
生物
斑马鱼
毒性
发育毒性
静脉丛
人口
脐静脉
解剖
药理学
毒理
男科
内科学
胎儿
生物化学
杀虫剂
胚胎干细胞
医学
基因
遗传学
生态学
体外
环境卫生
怀孕
作者
Yan Ma,Qicheng Zhu,Shili Luo,Fenghong Zhang,Lei Liu,Zhi Mengxue,Zhuyi Zhang,Xiaolian Cao,Xuelin Qiu,Zeng Xiang-yu,Di Ji,Chenxin Li,Xingwu Zhong,Jianshe Wang,Yanhong Wei
出处
期刊:Chemosphere
[Elsevier]
日期:2023-09-01
卷期号:335: 139146-139146
被引量:2
标识
DOI:10.1016/j.chemosphere.2023.139146
摘要
The pesticide fipronil is widely dispersed in aquatic environments and frequently detected in the general population. Although the adverse effects on embryonic growth by fipronil exposure have been extensively documented, the early responses for its developmental toxicity are largely unknown. In the present study, we explored the sensitive targets of fipronil, focusing on vascular injury using zebrafish embryos/larvae and cultured human endothelial cells. Exposure to 5–500 μg/L fipronil at the early stage impeded the growth of sub-intestinal venous plexus (SIVP), caudal vein plexus (CVP), and common cardinal veins (CCV). The damages on venous vessels occurred at exposure to the environmentally relevant concentration as low as 5 μg/L fipronil, whereas no significant change was observed in general toxicity indexes. In contrast, vascular development of the dorsal aorta (DA) or intersegmental artery (ISA) was not affected. In addition, the mRNA levels of vascular markers and vessel type-specific function genes exhibited significant decreases in venous genes, including nr2f2, ephb4a, and flt4, but no appreciable change in arterial genes. Likewise, the more pronounced changes in cell death and cytoskeleton disruption were shown in human umbilical vein endothelial cells as compared with human aortic endothelial cells. Furthermore, molecular docking supported a stronger affinity of fipronil and its metabolites to the proteins correlated with venous development, such as BMPR2 and SMARCA4. These results reveal the heterogeneity in developing vasculature responsive to fipronil's exposure. The preferential impacts on the veins confer higher sensitivity, allowing them to be appropriate targets for monitoring fipronil's developmental toxicity.
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