BACKGROUND: A comprehensive evaluation of basic motor abilities is provided by the Test of Gross Motor Development-3 (TGMD-3), which assesses 13 basic motor skills. These skills are categorized into locomotor and ball skill subsets. OBJECTIVE: An assessment of gross motor skills in diverse populations with the TGMD-3 is the goal of this study. METHODS: From control and intervention groups, locomotor subset scores were collected. In addition to identifying developmental delays, the study highlights the TGMD-3's ability to detect deficits in motor skills. Depending on whether a skill meets the criteria, it is scored as either a 1 or a 0. A locomotor score of 46, a ball skill score of 54, and an overall gross motor score of 100 are the maximum scores. RESULTS: The control group's baseline scores increased from 34.3±0.9 to 37.3±0.7 by the sixth week (p = 0.03), while the intervention group's scores rose from 36.5±1.1 to 40.9±0.6 (p = 0.0007). Both groups also showed similar trends in gross motor coordination scores. CONCLUSION: Gross motor skill assessment is robust across different populations, making the TGMD-3 an effective tool for improving motor development and performance.