This paper examines the micromixing process in a square-wave-based micromixer to mix deionized water and plasma for biological applications. The numerical simulations are carried out using COMSOL Multiphysics software by utilizing the finite element method (FEM). The performance of three types of square-wave-based micromixer are assessed and it is demonstrated that the micromixer Type III with the most number of cylindrical obstacles in the flow direction has the maximum mixing index. The results reveal that as the inlet velocity is reduced, the values of the mixing index are augmented due to an increment in the residence time of liquids in the micromixer.