体内
生物分析
酯酶
生物传感器
分子探针
临床前影像学
合理设计
纳米技术
化学
生物化学
生物
酶
材料科学
生物技术
DNA
作者
Wu‐Yingzheng Guo,Yi‐Xuan Fu,Long‐Can Mei,Zhao Chen,Zi-Ye Zhang,Fan Wang,Wen‐Chao Yang,Guozhen Liu,Guang‐Fu Yang
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2023-05-05
卷期号:8 (5): 2041-2049
被引量:1
标识
DOI:10.1021/acssensors.3c00264
摘要
Small-molecule fluorogenic probes are indispensable tools for performing research in biomedical fields and chemical biology. Although numerous cleavable fluorogenic probes have been developed to investigate various bioanalytes, few of them meet the baseline requirements for in vivo biosensing for disease diagnosis due to their insufficient specificity resulted from the remarkable esterase interferences. To address this critical issue, we developed a general approach called fragment-based fluorogenic probe discovery (FBFPD) to design esterase-insensitive probes for in vitro and in vivo applications. With the designed esterase-insensitive fluorogenic probe, we successfully achieved light-up in vivo imaging and quantitative analysis of cysteine. This strategy was further extended to design highly specific fluorogenic probes for other representative targets, sulfites, and chymotrypsin. The present study expands the bioanalytical toolboxes available and offers a promising platform to develop esterase-insensitive cleavable fluorogenic probes for in vivo biosensing and bioimaging for the early diagnosis of diseases.
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