This study systematically assessed chemical and physical hazards in 28 educational laboratories at the Qazvin University of Medical Sciences using the assessment and classification of hazards in laboratories (ACHiL) framework. The assessment involved walkthrough inspections, interviews, and checklist-based evaluations aligned with the guidelines of the Occupational Safety and Health Administration (OSHA), Globally Harmonized System (GHS) for Classification and Labeling of Chemicals, and National Fire Protection Association (NFPA). Among the 540 chemicals analyzed, 57% were classified as high risk, including substances such as methanol, phenol, nitric acid, and sulfuric acid. The Toxicology, Environmental Health, and Microbiology laboratories showed the highest concentrations of hazardous chemicals. Physical hazards, including electrical risks, ultraviolet radiation, and hot surfaces, were also identified and classified based on WHO and NFPA protocols. The results underscore the urgent need for enhanced chemical safety training, consistent use of personal protective equipment (PPE), improved ventilation systems, and regular inventory updates. This methodology offers a replicable model for laboratory risk assessment and contributes to the advancement of occupational health and safety in academic environments.