Brown Adipose Tissue Transplantation

The metabolic benefits of brown adipose tissue (BAT) are well known. Increasing the BAT content and/or activity is a proposed therapeutic approach to combat metabolic disease. Activation and induction of endogenous BAT have achieved varying degrees of success in correcting obesity, insulin resistance, and cardiovascular disease, with some limitations. Transplantation of BAT from healthy donors is another approach proven safe and effective in rodent models. In diet-induced models of obesity and insulin resistance, BAT transplants prevent obesity, increase insulin sensitivity, and improve glucose homeostasis and whole-body energy metabolism. In mouse models of insulin-dependent diabetes, subcutaneous transplantation of healthy BAT produces long-term euglycemia without the need for insulin or immunosuppression. Considering the immunomodulatory and anti-inflammatory properties of healthy BAT, transplantation may be a more effective approach to combat metabolic disease in the long term. Here we describe in detail the technique for subcutaneous BAT transplantation.