Establishing a growth-coupled mechanism for high-yield production of β-arbutin from glycerol in Escherichia coli

Biodiesel production has increased significantly in recent years, leading to an increase in the production of crude glycerol. In this study, a novel growth-coupled erythrose 4-phosphate (E4P) formation strategy that can be used to produce high levels of β-arbutin using engineered Escherichia coli and glycerol as the carbon source was developed. In the strategy, E4P formation was coupled with cell growth, and a growth-driving force made the E4P formation efficient. By applying this strategy, efficient microbial synthesis of β-arbutin was achieved, with 7.91 g/L β-arbutin produced in shaking flask, and 28.1 g/L produced in a fed batch fermentation with a yield of 0.20 g/g glycerol and a productivity of 0.39 g/L/h. This is the highest β-arbutin production through microbial fermentation ever reported to date. This study may have significant implications in the large-scale production of β-arbutin as well as other aromatic compounds of importance.