A portable hydrogel kit based on Au@GM88A/I combined with mobile phone for polychromatic semi-quantitative and quantitative sensing analysis

The development of an affordable, portable, and instrument-free colorimetric biosensor holds significant importance for routine monitoring and clinical diagnosis. To overcome the limitations that traditional monochromatic colorimetric kits struggle to distinguish subtle color changes with the naked eye, we designed and constructed a portable hydrogel kit for polychromatic semi-quantitative and quantitative sensing analysis. When the actual samples and Iˉ were introduced into a gelatin hydrogel encapsulated with MIL-88A(Fe), Au NRs and oxidase (Au@GM88A/I), a noticeable color change occurred. Additionally, a mathematic model between Hue and multicolor signal was set up for the first time by mobile phone photo technology, successfully applied to the glucose detection in serum. The visual detection had a wide concentration range of 0.02–0.80 mM with a limit of detection down to 0.02 mM. Above all, hydrogel kit prepared with gelatin as a carrier addressed the issues of uneven color and slow response rate commonly seen in gels like sodium alginate and agarose. This improvement would be beneficial for enhancing the accuracy of color captured by mobile phone assisted hydrogel kits, making it a valuable tool for biomarker analysis.