组织工程
自愈水凝胶
细胞生物学
再生医学
诱导多能干细胞
作者
Wen-Ning Lu,Shuanghong Lü,Haibin Wang,Dexue Li,Cuimi Duan,Zhiqiang Liu,Tong Hao,Wenjun He,Bin Xu,Qiang Fu,Ying C. Song,Xiao-Hua Xie,Changyong Wang
出处
期刊:Tissue Engineering Part A
[Mary Ann Liebert]
日期:2009-06-01
卷期号:15 (6): 1437-1447
被引量:189
标识
DOI:10.1089/ten.tea.2008.0143
摘要
Transplantation of embryonic stem cells (ESCs) can improve cardiac function in treatment of myocardial infarction. The low rate of cell retention and survival within the ischemic tissues makes the application of cell transplantation techniques difficult. In this study, we used a temperature-responsive chitosan hydrogel (as scaffold) combined with ESCs to maintain viable cells in the infarcted tissue. Temperature-responsive chitosan hydrogel was prepared and injected into the infarcted heart wall of rat infarction models alone or together with mouse ESCs. The result showed that the 24-h cell retention and 4 week graft size of both groups was significantly greater than with a phosphate buffered saline control. After 4 weeks of implantation, heart function, wall thickness, and microvessel densities within the infarct area improved in the chitosan + ESC, chitosan, and ESC group more than the PBS control. Of the three groups, the chitosan + ESC performed best. Results of this study indicate that temperature-responsive chitosan hydrogel is an injectable scaffold that can be used to deliver stem cells to infarcted myocardium. It can also increase cell retention and graft size. Cardiac function is well preserved, too.
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