Studies on the cellulase production using corncob residue from xylose manufacture by trichoderma sp.B-8 and the characterization of the cellulase

To improve enzymatic hydrolytic efficiency and reduce production cost, the cellulase production by Trichoderma sp. B-8, isolated from corncob residue from xylose manufacture, was investigated, using corncob reside as main carbon source. The optimal carbon and nitrogen sources used in the fermentation medium were as follows: corncob residues 2.5%, wheat bran 0.75%, peptone 0.5% and corn steep liquor 2.0%. The optimal hydrolysis temperature and pH of the cellulase were 45°C and 5.0, respectively. The activities of FPAase and CMCase of the crude cellulase liquor determined at the optimal hydrolysis temperature and pH reached 3.32IU/ml and 25.7IU/ml, respectively. In addition, the most important, the glucose production by saccharifying corncob residue using crude cellulase was as high as 41.0g/L on the 10% (W/V) corncob residues base.