介电谱
循环伏安法
化学
胶体金
亲和素
电极
电化学
无机化学
银纳米粒子
纳米颗粒
化学修饰电极
参比电极
材料科学
纳米技术
生物素化
生物化学
物理化学
作者
Nirgaman Bage,Pradip Kar
标识
DOI:10.1080/10739149.2020.1787439
摘要
A gold electrode was modified with cysteamine by a self-assembly monolayer technique and covalently coupled with 3-mercaptopropionic acid functionalized silver nanoparticles. The free carboxylic acid groups of the 3-mercaptopropionic acid functionalized silver nanoparticles assembled on gold electrode were bonded with the amine groups of biotin or avidin trough amide linkages. The changes in the interfacial properties of gold electrode modified stepwise were examined by cyclic voltammetry and electrochemical impedance spectroscopy using the ferric/ferrous redox system. The electrochemical sensing properties of covalently bonded biotin or avidin on a gold electrode modified with silver nanoparticle were studied by cyclic voltammetry and electrochemical impedance spectroscopy by the varying concentrations of the bio-affinity molecules in the same redox system. The charge transfer resistance was directly proportional to the concentration of analyte while the response was weakly dependent upon the change in the respective peak anodic currents. The sensing mechanism was established to explain the role of the silver nanoparticles.
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