化学
生物分子
DNA
纳米技术
连锁反应
生物传感器
分支迁移
生物物理学
DNA–DNA杂交
信号(编程语言)
杂交探针
基因分型
A-DNA
DNA纳米技术
生物系统
霍利迪路口
生物化学
DNA修复
基因
计算机科学
生物
材料科学
光化学
程序设计语言
基因型
作者
Bingling Li,Yu Jiang,Xi Chen,Andrew D. Ellington
摘要
Catalyzed hairpin assembly (CHA) is a robust enzyme-free signal-amplification reaction that has a wide range of potential applications, especially in biosensing. Although most studies of the analytical applications of CHA have focused on the measurement of concentrations of biomolecules, we show here that CHA can also be used to probe the spatial organization of biomolecules such as single-stranded DNA. The basis of such detection is the fact that a DNA structure that brings a toehold and a branch-migration domain into close proximity can catalyze the CHA reaction. We quantitatively studied this phenomenon and applied it to the detection of domain reorganization that occurs during DNA self-assembly processes such as the hybridization chain reaction (HCR). We also show that CHA circuits can be designed to detect certain types of hybridization defects. This principle allowed us to develop a “signal on” assay that can simultaneously respond to multiple types of mutations in a DNA strand in one simple reaction, which is of great interest in genotyping and molecular diagnostics. These findings highlight the potential impacts of DNA circuitry on DNA nanotechnology and provide new tools for further development of these fields.
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