[Expression of recombinant fibronectin FNIII8-10 in Pichia pastoris and fermentation process optimization].

) methanol supplementation. Meanwhile, with sorbitol as an auxiliary carbon source to alleviate methanol stress, the yield of the recombinant FNIII8-10 reached 65.49 mg/L. Finally, high-cell-density fermentation achieved a yield of 532.82 mg/L, representing a 7-fold increase compared with the highest yield in shake flasks. This study lays a technical foundation for the industrial-scale production of recombinant FNIII8-10.