Oligodendrocyte–Neuron Myelinating Coculture

The preparation of oligodendrocytes and neurons independently in vitro has provided substantial insight into the biology of the process of myelin sheath formation. This chapter describes a myelination system of dorsal root ganglion neurons by independent isolation of oligodendrocyte progenitor cells from either rat or mouse cortex. This in vitro assay can be used to examine the molecular determinants of myelin sheath formation.