重组DNA
生物反应器
生物化学
产量(工程)
酶
生物量(生态学)
大肠杆菌
功能(生物学)
生物
化学
细胞生物学
植物
基因
材料科学
农学
冶金
作者
Kaisa Ukkonen,Antje Neubauer,Vinit J. Pereira,Antti Vasala
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 127-137
被引量:7
标识
DOI:10.1007/978-1-4939-6887-9_8
摘要
Expression of recombinant proteins in sufficient quantities is essential for protein structure-function studies. The most commonly used method for recombinant protein production is overexpression in E. coli cultures. However, producing high yields of functional proteins in E. coli can be a challenge in conventional shaken cultures. This is often due to nonoptimal growth conditions, which result in low cell yields and insoluble or incorrectly folded target protein. To overcome the shortcomings of shake flask cultivation, we present a cultivation method based on enzymatic glucose delivery. This system mimics the fed-batch principle used in bioreactor cultivations and provides high yields of biomass and recombinant proteins in shaken cultivations.
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