Effects of salidroside and its derivatives on the radicals by EPR method

OBJECTIVE To research salidroside and it s derivatives on the hydroxy radicals (OH·) and superoxide anion radicals (O -· 2)in vitro.METHOD ①OH· generated in Fenton reaction and O - · 2 generated in the alkaline dimethyl sulfoxide were used as model systems respectively, and signals of spin trapping adducts (DMPO-OH·) and O -· 2were measured by electron paramagnetic resonance (EP R). The effects of salidroside and its derivatives on EPR signals of adducts wer e observed. RESULTS The eliminating effect of m-hydroxyphenethyl-β- D-galactoside on OH· and O -· 2 was stronger than the ones of salidroside and other derivatives. CONCLUSION The length of carbochain connecting benzene ring, hy droxyl group and its position in benzene ring and the types of saccharide group connecting aglucon were major factors on the eliminating effects of salidroside and its derivatives for OH·and O -· 2,and the type of saccharide group connecting aglucon is the most important factor.