üRP-HPLC analysis of paeoniflorin,ferulaic acid,naringin and glycyrrhizic acid in Xuefu Zhuyu granules

Objective:To develop an HPLC method for determination of paeoniflorin,ferulaic acid,naringin and glycyrrhizic acid in Xuefu Zhuyu granules.Methods:The analysis was carried out on an Sepax C18(250 mm×4.6 mm,5 μm)column;The mobile phase was composed of 0.1% phosphoric acid aqueous(A)and 70% acetonitrile(B)with gradient elution(0-30 min,24% B→40% B;30-50 min,40% B→100% B;51-60 min,24% B)and the flow rate was 1.0 mL·min-1;The detection wavelengths were set at 230 nm for paeoniflorin(0-12 min),320 nm for ferulaic acid(12.1-15.5 min)and 283 nm for naringin(15.6-30 min)and 250 nm for glycyrrhizic acid(30.1-60 min);The column temperature was 40 ℃.Results:The linear ranges of paeoniflorin,ferulaic acid,naringin and glycyrrhizic acid were 0.05-0.65 μg,0.01-0.13 μg,0.05-0.65 μg,0.02-0.28 μg(r≥0.9990,respectively);The average recoveries were 101.9%,104.3%,104.4% and 102.9%(RSD2.5%,n=6,respectively).Conclution:This method is simple and can be used to determine the above 4 components with satisfactory accuracy and repeatability.