Susceptibility of Soybean Cotyledonary Node to Kanamycin

[Objective] To study the susceptibility of soybean cotyledonary node to kanamycin,so as to provide experimental basis for the genetic transformation and screening of soybean.[Method] With three different genotypes of soybean as test materials,their yellowing rate and differentiation rate were measured to study the impact of different concentration of kanamycin on the differentiation and shoots growth of soybean cotyledonary node.[Result] Different genotypes of soybean showed different degree of susceptibility to kanamycin,and the critical concentration of kanamycin for the differentiation of cotyledonary was 40 mg/L to Kennong 18,60 mg/L to Suinong 14 and Kennong 4;while the critical concentration of kanamycin for the rooting of shoots was 15 mg/L to Kennong 18,20 mg/L to Suinong 14 and Kennong 4.[Conclusion] The study laid foundation for the genetic transformation and screening of soybean.