Objective: To screen the extracting method of utility portion in Carthamus tinctorius. Methods: A RP-HPLC method was developed to determinate the content of hydroxysafflor yellow A in extracting portin of safflor yellow. According to the mono factor design and uniform design. We screened the extracting method of utility portion in Carthamus tinctorius. Results: The extracting solvent was water, repeat 2 times, every time add 13 times water and the extracting lasts 20 min to the Chinese medicine drug. The content of hydroxysafflor yellow A was highest in extraction for every gram Carthamus tinctorius. Conclusion: The method is simple, stabilization and feasible.