Determination of the strength and the biocompatibility of APA microcapsule membrane

Objective:To know the biocompatibility of APA microcapsules we made and compare the difference in the mechanical strength of two kind of capsules.Methods:The alginate poly L lysine alginate (APA) microcapsules were made by the static generator The microcapsules were divided into typeⅠor typeⅡ according to using sodium citron to liquefy bead core or not The mechanical strength of the microcapsules was measured by shaking method The chemical intensity of APA microcapsules was detected by using vary acerbity and alkalinity liquor The biocompatibility of APA microcapsules was observed by transplanting them into intraperitoneum of mice.Results:The broken ratio of 48h mechanism shaking: type Ⅰ5% and type Ⅱ31%,which indicated that type Ⅰhad better mechanical strength than type Ⅱ had 2 pH7 4 were suitable for the microcapsules 3 The microcapsules were taken out from mice intraperitoneum after transplanted 1、2、4、8 and 10 weeks, and 95 98 percent of these capsules were of integrity, rotundity, surface smooth, without obviously bundled by connective tissue. Conclusion:The type Ⅰ APA microcapsules we made had good biocompatibility、mechanical strength and chemical intensity.