The present study investigated the differences of DEHP accumulation in peel and pulp of the same breed of different batches of Guangdong black peel wax gourd(Benincasa hispida(Thunb.)Cogn.)fruit by gas chromatography/mass spectrometry(GC/MS),meanwhile,we analyzed waxy composition in DEHP accumulation of the maximum and minimum batches of wax gourd.The results indicated that the peel of wax gourd accumulated more DEHP than pulp,and all batches of wax gourd were statistically significant(P0.01),DEHP accumulated from(12.603±0.7860) mg/kg to(37.628±1.7326) mg/kg(fresh weight,FW)in the peel of wax gourd,while DEHP accumulated from(3.642±0.2010) mg/kg to(6.474±0.1831) mg/kg in the pulp of wax gourd only.Short retention time wax appeared more in low accumulative batch of wax gourd,and long retention time wax appeared more in high accumulative batch of wax gourd.Present investigation showed that differences of waxy composition may lead to the accumulation of DEHP differences in wax gourd.