Effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelial cells

Objective: To observe the effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelia cells (HUVEC). Methods: Primary and passaged HUVEC were cultured in the medium with 20% mixed umbilical cord blood plasma and their purities were evaluated by Ⅷ:Ag and UEA Ⅰ.Proliferative activities of HUVEC were compared in the medium containing different amounts of mixed umbilical cord blood plasma by methyl thiazolyl tetrazolium(MTT) assay. Results: 20% mixed umbilical cord blood plasma can maintain cell viability in the medium without endothelial cell growth factor(ECGF). The purity of endothelial cells was 93.1%. Conclusion: 20 portion mixed umbilical cord blood plasma can be a substitute for ECGF and used successfully in the culture of HUVEC to obtain high purity and passagable endothelial cells.