Determination of aflatoxin B_1 in peanuts by liquid chromatography-tandem mass spectrometry

An analytical method for the determination of aflatoxin B1 in peanuts by high performance liquid chromatography-tandem mass spectrometry has been developed.This article focused on the optimization of extraction,clean-up,HPLC separation and MS/MS parameters of the analyte.Aflatoxin B1 was extracted by 60%(V/V) of methanol aqueous solution,and then it was purified by OASIS HLB SPE column.The eluted extract was analyzed by HPLC-MS/MS in positive ion mode using multiple reaction monitoring with a triple-quadruple mass spectrometer using an electrospray ionization source.An isocratic mobile phase composed of methanolwater(70/30)(V/V) was used,water with 0.1% formic acid(V/V).With the precursor ion =313.0 and the product ion =241.1、 269.1,the retention time of aflatoxin B1 was 3.30 min.Under the ESI+ analysis,a high correlation coefficients(0.999) of aflatoxin B1 was obtained within linear range(0.1～50 μg/kg).Sample recoveries at three spiking levels ranged from 93% to 105%(relative standard deviation(RSD≤5%).Determination limit(S/N=3) was 0.03 μg/kg and quantification limit(S/N=10) was 0.1 μg/kg.