Detection of the expression of hypoxia-inducible factor 1 alpha in gastric mucosal disease by using tissue chip technique

Objective To study the significance of expression of hypoxia-inducible factor 1 alpha(HIF-1α) in gastric mucosal diseases. Methods Using tissue chip technique to creat tissue chip in 120 cases of vary gastric mucosal disease,and using S-P immunohistochemical methods to detect the expressions of HIF-1α in these tissue chips. Results The positive rates of HIF-1α was 28.3% in 120 tissue chips of gastric mucosal diseases. The positive rates of HIF-1α was 0, 10.0%, 40.0% and 63.3% in chronic superficial gastritis, chronic atrophic gastritis with intestinal metaplasia, chronic atrophic gastritis with dysplasia and intestinal type gastric cancer, respectively. The positive rates of HIF-1α of intestinal type gastric cancer and chronic atrophic gastritis with dysplasia were significantly higher than that in chronic superficial gastritis and chronic atrophic gastritis with intestinal metaplasia(P0.05). Conclusions The results demonstrate that HIF-1α is a good marker of gastric precancerous lesion. It is feasible to use of tissue chip for a rapid screen of massive tissue specimens clinically,and it is a high efficacy,economic and accurate method for delecting gastric precancerous lesion.