77 endophytic fungi strains were isolated from gingkgo.The Botrytis cinerea which was the main pathogenic fungus of the crop was chosen as indication fungus to screen antagonistic fungi.Antagonistic cultivation showed that 46 strains had antagonism and accounted for 59.7% of all fungi;7 strains showed stronger antagonism than others and accounted for 9.1% of all fungi.Through a series of tests,strains 2-18 and 2-9(Chaetomium sp.) were screened out which had stable inhibiting effect to Botrytis cinerea,and the inhibiting rate was between 80.8% and 100%.