Decolorization effects on polysaccharide from Acaudina molpadioides using activated carbon,H2O2 and macroporous resins were compared.From respects of decolorization rate and polysaccharide retention rate to consider,macroporous resin D392 selected from 8 types of macroporous resins was better than activated carbon and H2O2.Response surface methodology was adopted to optimize the conditions on the basis of single factor experiments for investigating the maximum decoloration effect.A second order quadratic equation was established.The optimal decolorization technology was obtained,including time of 5 hours,pH 10,and temperature of 60℃.The decolorization rate was(84.2±0.6)%,and the polysaccharide reserved rate was(82.9±1.1)%.It could provide well guarantee for subsequent application research of sea cucumber polysaccharide.