Lectin from Bradybaena similaris's Agglutination Activity and Its Influencing Factors

The agglutination activity of albumen gland extract from a Bradybaena similaris was measured.The agglutinin was extracted and part of its properties was identified.The results showed agglutinin existed in the albumen gland of bradybaena similaris;this agglutinin had blood-cell agglutination activity,and was able to agglutinate 9 kinds of animal red blood-cells,of which the domestic rabbit's was agglutinated with highest activity up to 28.However,the agglutination activity on domestic rabbit's red blood-cell was significantly dependent on Ca2+.It was stable in pH 7.0~8.0 with 100 % activity,and was lost after continuous functioning for 10 minutes in 40 ℃ temperature,which indicated its heat resistance was poor.45 % solid ammonium sulfate was added into the albumen gland extract from a bradybaena similaris to precipitate the agglutinin,and then the precipitates were extracted respectively by using ammonium sulfate solution with different saturation.The specific activity of the extract was increased by about 2.56 times with total activity recovery of 20.00%.