Glycosyl-linkages of Acid Soluble Polysaccharide from Green Laver, Enteromopha prolifera

국내에서 양식되는 가시파래의 당 결합 양식을 조사하기 위하여 가시파래의 수용성 산성 다당을 황산기 제거 및 우론산의 환원처리를 한 후 methylation하여 GC/MSD로 분석을 하였다. 가시파래의 수용성 산성 다당의 우론산은 대부분이 glucuronic acid 였으며, 주로 황산기를 함유한 rhamnose, xylose, glucuronic acid로 이루어진 다당이었다. 이 수용성 산성 다당을 화학적 수식한 후 GC/MSD로 분석시 황산기는 rhamnose의 C-3과 xylose의 C-2에 결합되어 있었으며, 주 결합구조는 C-3에 황산기가 결합한 1,4-, 1,2,4-결합된 rhamnose, C-2에 황산기가 결합한 1,4-결합된 xylose, 1,4-결합된 xylose, 그리고 1,4-결합된 glucuronic ac:김였다. 그리고 일부 미량의 1,4-결합된 rhamnose, 1,4,6-결합된 galactose도 분리되었다. Green layer, Enteromorpha prolifera, is regarded as one of important materials for food processing in Korea. The acidic water-soluble polysaccharide (CPC-PS) isolated from the alga with hot water and cetylpyridium chloride was mainly constituted of rhamnose, xylose, uronic acid and sulfate. To determine the glycosyl-linkages and positions of sulfate by methylation, the CPC-PS was reduced and/or sulfates. A marked increase of glucose content in the reduced polysaccharide indicated that glucuronic acid was a major sugar in the polymer and sulfation was deduced to occur on O-3 of rhamnose and O-2 of xylose. According to the methylation analysis of the native, reduced, desulfated and reduced-desulfated polymers, CPC-PS mainly composed of 1,4- and 1,2,3-linked rhamnose 3-sulfate, 1,4-linked xylose 2-sulfate, 1,4-linked xylose and 1,4-linked glucuronic acid. Minor 1,4-linked rhamnose and 1,4,6-linked galactose residues were also detected.