聚丙烯酰胺凝胶电泳
凝胶电泳
权重估算
色谱法
蛋白质凝胶电泳
化学
电泳
聚丙烯酰胺
功率(物理)
生物化学
数学
酶
统计
物理
量子力学
高分子化学
作者
Arnold L. Shapiro,Jacob V. Maizel
标识
DOI:10.1016/0003-2697(69)90335-2
摘要
Abstract Molecular weights of polypeptides and proteins can be determined with a high degree of accuracy by electrophoresis in SDS-polyacrylamide gels. Coelectrophoresis of dual-labeled markers and unknowns permits resolution of molecular weight differences as small as 5% (and possibly 2%) between polypeptides. Experiments with S35-labeled SDS suggest that the SDS-protein complex is a loose one and that exchange of SDS with the environment occurs at an exceedingly high rate.
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