聚合酶链反应
计算生物学
生物
重组DNA
聚合酶
聚合酶链反应优化
鉴定(生物学)
分子生物学
遗传学
基因
多重聚合酶链反应
植物
作者
Henry A. Erlich,David H. Gelfand,John J. Sninsky
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1991-06-21
卷期号:252 (5013): 1643-1651
被引量:994
标识
DOI:10.1126/science.2047872
摘要
The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.
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