生物
细胞周期蛋白依赖激酶1
细胞周期蛋白B
细胞周期蛋白D
细胞周期蛋白A2
细胞周期蛋白依赖激酶复合物
细胞周期蛋白
促成熟因子
细胞生物学
细胞周期蛋白依赖激酶
细胞周期蛋白
分子生物学
细胞周期蛋白依赖激酶2
蛋白激酶A
生物化学
细胞周期
激酶
细胞
作者
Giulio Draetta,Frank Luca,Joanne M. Westendorf,Leonardo Brizuela,Joan V. Ruderman,David Beach
出处
期刊:Cell
[Elsevier]
日期:1989-03-01
卷期号:56 (5): 829-838
被引量:648
标识
DOI:10.1016/0092-8674(89)90687-9
摘要
In the clam, Spisula, two previously described proteins known as cyclin A and B display the unusual property of selective proteolytic degradation at the end of each mitosis. We show here that clam oocytes and embryos contain a cdc2 protein kinase. This protein kinase is a component of the M phase promoting factor (MPF) in frog eggs and the M phase-specific histone H1 kinase in starfish. Clam cdc2 is found in association with both cyclin A and B, probably not as a trimolecular association, but as separate cdc2/cyclin A and cdc2/cyclin B complexes. Clam cdc2 and the associated cyclins bind to p13suc1-Sepharose. The p13-bound complex, and also anti-cyclin A or B immunoprecipitates, each display cell cycle-dependent histone H1 kinase activity. We suggest that in addition to the cdc2 protein kinase, the cyclins are further components of the M phase promoting factor and that cyclin proteolysis provides the mechanism of MPF inactivation and thus exit from mitosis.
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