牙乳头
成牙本质细胞
波形蛋白
细胞角蛋白
细胞生物学
化学
鬼臼苷
牙髓(牙)
牙囊
解剖
病理
生物
间充质干细胞
细胞骨架
免疫组织化学
医学
细胞
生物化学
作者
P. P. Webb,B.J. Moxham,J. R. Ralphs,Michael Benjamin
标识
DOI:10.3109/03008209509013708
摘要
This study describes the immunolocalization of actin, cytokeratins and vimentin during differentiation of the dental papilla in the rat. Incisors and first molars were sectioned from mandibles of Wistar rats from embryonic day (E)-14 to (E)-21 and weeks 1, 2, 3,12 and 104 after birth, fixed in 90% alcohol, decalcified in EDTA, infiltrated with 5% sucrose, frozen in dry ice, and cryosectioned at 10 μm. The sections were immunolabelled using indirect immunofluorescence with a panel of monoclonal antibodies and FITC-phalloidin for F-actin localization. F-actin was present in follicular mesenchyme and odontoblast processes. Vimentin labelled dental papilla fibroblasts, differentiating, functional (secretory) and aged odontoblasts. Vimentin was uniformly localized in the cytoplasm of pre-odontoblasts but was redistributed to the apical pole of these cells during polarization. Of the cytokeratins, only cytokeratin 19 was found in differentiating odontoblasts. It was not present in dental papilla fibroblasts, functional or aged odontoblasts. These results suggest that actin and the redistribution of vimentin may be involved in odontoblast differentiation and odontoblast process formation/ support and that these events may be preceded by the expression of cytokeratin 19.
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