细胞生物学
壁细胞
磷酸戊糖途径
细胞外基质
斑马鱼
生物
弹性蛋白
氧化应激
内皮干细胞
生物化学
化学
新陈代谢
糖酵解
体外
遗传学
基因
作者
Nicola Facchinello,Matteo Astone,Matteo Audano,Roxana E Oberkersch,Marianna Spizzotin,Enrica Calura,Madalena Marques,Mihaela Crisan,Nico Mitro,Massimo Santoro
标识
DOI:10.1038/s42255-021-00514-4
摘要
Vascular mural cells (vMCs) play an essential role in the development and maturation of the vasculature by promoting vessel stabilization through their interactions with endothelial cells. Whether endothelial metabolism influences mural cell recruitment and differentiation is unknown. Here, we show that the oxidative pentose phosphate pathway (oxPPP) in endothelial cells is required for establishing vMC coverage of the dorsal aorta during early vertebrate development in zebrafish and mice. We demonstrate that laminar shear stress and blood flow maintain oxPPP activity, which in turn, promotes elastin expression in blood vessels through production of ribose-5-phosphate. Elastin is both necessary and sufficient to drive vMC recruitment and maintenance when the oxPPP is active. In summary, our work demonstrates that endothelial cell metabolism regulates blood vessel maturation by controlling vascular matrix composition and vMC recruitment.
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