检出限
化学
化学发光
钌
辣椒素
电化学发光
线性范围
电化学
缓冲溶液
分析物
玻璃碳
特里斯
电极
无机化学
分析化学(期刊)
色谱法
物理化学
有机化学
循环伏安法
催化作用
受体
生物化学
作者
Sang Jung Lee,Won Young Lee
标识
DOI:10.1016/j.jelechem.2022.116169
摘要
A highly sensitive tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)32+] electrogenerated chemiluminescence (ECL) detection method for the determination of capsaicin has been developed. In this study, capsaicin was worked as a coreactant in the oxidative-reduction pathway of the Ru(bpy)32+ ECL. The oxidation of Ru(bpy)32+ together with coreactant capsaicin on a bare glassy carbon electrode at an applied potential of + 1.10 V vs. Ag/AgCl (3 M NaCl) led to a strong ECL emission. The Ru(bpy)32+ ECL emission of capsaicin was strongly dependent upon the buffer solution pH, in which a strong ECL was obtained in alkaline buffer solution. Under the optimized conditions, the present Ru(bpy)32+ ECL detection method showed a linear response to capsaicin in the linear range from 1.0 × 10-7 M to 1.0 × 10-4 M with a limit of detection of 9.4 × 10-8 M (S/N = 3). In addition, the present method can detect dihydrocapsaicin and nordihydrocapsaicin with a detection limit of 1.9 × 10-7 M and 2.2 × 10-7 M, respectively. The present ECL method shows good analytical performance for capsaicinoids in terms of linear dynamic range and detection limit compared to those obtained at other electrochemical methods even without the employment of additional modification of electrode surfaces to enhance the detection responses. In addition, no analyte preconcentration was required. The recovery tests performed for spiked capsaicin in real pepper samples were satisfactory with the recovery from 95% to 103%. To the best of our knowledge, this is the first report on the application of ECL for the quantitative determination of capsaicinoids. Since the present method is very simple, fast and sensitive, it could be applied for the determination of capsaicin in food and pharmaceuticals.
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