The SpliZ generalizes ‘percent spliced in’ to reveal regulated splicing at single-cell resolution

Detecting single-cell-regulated splicing from droplet-based technologies is challenging. Here, we introduce the splicing Z score (SpliZ), an annotation-free statistical method to detect regulated splicing in single-cell RNA sequencing. We applied the SpliZ to human lung cells, discovering hundreds of genes with cell-type-specific splicing patterns including ones with potential implications for basic and translational biology. The Splicing Z Score (SpliZ) provides single-cell-level quantification of alternative splicing with improved statistical power.