计算生物学
有机体
鉴定(生物学)
蛋白质组学
系统生物学
生物
蛋白质-蛋白质相互作用
计算机科学
纳米技术
生化工程
生物化学
遗传学
工程类
生态学
材料科学
基因
作者
Xinxin Yang,Zhiyan Wen,Dingliang Zhang,Zhen Li,Dawei Li,Ugrappa Nagalakshmi,Savithramma P. Dinesh-Kumar,Yongliang Zhang
标识
DOI:10.1016/j.xplc.2020.100137
摘要
Protein-protein interaction (PPI) networks are key to nearly all aspects of cellular activity. Therefore, the identification of PPIs is important for understanding a specific biological process in an organism. Compared with conventional methods for probing PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach for characterizing PPIs. However, the application of PL in planta remains in its infancy. Here, we summarize recent progress in PL and its potential utilization in plant biology. We specifically summarize advances in PL, including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.
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