紫杉醇
生物化学
酶
水飞蓟
松柏醇
代谢工程
异源的
异源表达
过氧化物酶
乳蓟
化学
生物合成
生物
代谢途径
重组DNA
抗氧化剂
类黄酮
植物
药理学
基因
作者
Jiazeng Yang,Jincai Liang,Lingyun Shao,Lihong Liu,Ke Gao,Junliang Zhang,Zhenjiao Sun,Wendong Xu,Peng-Cheng Lin,Rongmin Yu,Jiachen Zi
标识
DOI:10.1016/j.ymben.2020.01.007
摘要
Silymarin extracted from milk thistle seeds, is used for treating hepatic diseases. Silybin and isosilybin are its main components, and synthesized from coupling of taxifolin and coniferyl alcohol. Here, the biosynthetic pathways of taxifolin and coniferyl alcohol were reconstructed in Saccharomyces cerevisiae for the first time. To alleviate substantial burden caused by a great deal of genetic manipulation, expression of the enzymes (e.g. ZWF1, TYR1 and ARO8) playing multiple roles in the relevant biosynthetic pathways was selectively optimized. The strain YT1035 overexpressing seven heterologous enzymes and five native enzymes and the strain YC1053 overexpressing seven heterologous enzymes and four native enzymes, respectively produce 336.8 mg/L taxifolin and 201.1 mg/L coniferyl alcohol. Silybin and isosilybin are synthesized from taxifolin and coniferyl alcohol under catalysis of APX1t (the truncated milk thistle peroxidase), with a yield of 62.5%. This study demonstrates an approach for producing silybin and isosilybin from glucose for the first time.
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