粘附
抗体
细胞粘附
微流控
细胞
化学
生物物理学
材料科学
分子生物学
纳米技术
生物
免疫学
生物化学
有机化学
作者
Akira Otaka,Atsushi Mahara,Kazuhíko Ishihara,Tetsuji Yamaoka
标识
DOI:10.1088/1361-6439/abe52a
摘要
Abstract Label-free cell separation is a promising method in the field of stem-cell research to obtain desired cell populations. Here, we report on phospholipid polymer-coated microfluidic channels with immobilized antibodies as devices for the capture of cells expressing target antigens in a label-free manner. We fabricated a microfluidic channel containing immobilized antibodies against vascular endothelial growth factor receptor 2 (Flk1), a potential marker for cardiac, angiogenic, and hematopoietic cell regeneration. A series of investigations was carried out to elucidate the effect of the immobilized antibodies on the adhesion behavior of the Flk1-expressing cell subpopulation derived from induced pluripotent stem cells. Increasing the immobilized antibody density (0.18–5.0 × 10 9 ligands mm −2 ) led to an increased number of cells adhering to the channel. The antibody-immobilized polymer-coated surface suppressed nonspecific cell adhesion, which was swept away by a weak shear flow, and captured Flk1-expressing cells under a wall shear stress of 1.7 Pa. Flk1 expression was 2.8-fold higher in the cells that adhered than in those that did not adhere. Therefore, an optimal antibody density and sweeping flow are required for effective label-free separation of Flk1-positive cells.
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